Description
General description
Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.
~ 34 kDa
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human Cyclin D at the C-terminus.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Cyclin D Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP to detect Cyclin D also known as B-cell CLL/lymphoma 1, BCL-1 oncogene.
Western Blot (SNAP ID) Analysis: 5 µg/ml from a previous lot detected Cyclin D on 10 µg of 3T3/A31 cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cyclin D in NIH/3T3, A431, C2C12, and HeLa cells.
Biochem/physiol Actions
This antibody recognizes Cyclin D at the C-terminus.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
3T3/A31 cell lysate
Evaluated by Western Blot in 3T3/A31 cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Cyclin D on 10 µg of 3T3/A31 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-221
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
