Description
General description
The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. STAT2 (113 kDa), originally purified from the nuclei of a-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta. Knockout mice with a targeted disruption of STAT2 exhibit greater susceptibility to viral infection and altered responses to Type I interferons. Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases.
~113 kDa observed. An uncharacterized band may appear at ~260 kDa in some lysates.
Immunogen
Epitope: Phosphorylated Tyr689.
KLH-conjugated linear peptide corresponding to mouse STAT2 phosphorylated at Tyr689.
Application
Anti-phospho-STAT2 (Tyr689) Antibody is an antibody against phospho-STAT2 (Tyr689) for use in Immunoprecipitation.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho-STAT2 (Tyr689) from IFN alpha treated HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Immunological Signaling
Biochem/physiol Actions
Other homologies: Rat (89% sequence homology).
This antibody recognizes STAT2 phosphorylated at Tyr689.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and IFN alpha/gamma treated HeLa cell lysate.
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:200 dilution of this antibody detected phospho-STAT2 (Tyr689) in 10 µg of IFN alpha/gamma treated HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
