Description
General description
Constitutive coactivator of PPAR-gamma-like protein 1, also known as Oxidative stress-associated Src activator or Protein FAM120A, and encoded by the gene name FAM120A or OSSA, was recently detected within the Purα-containing mRNA-protein complex in the brain. As a novel RNA-binding protein, FAM120A is a critical component of the oxidative stress-induced survival signaling. It may participate in mRNA transport in the cytoplasm. FAM120A activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. FAM120A is highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa. FAM120A protects cells from apoptosis through activation of SRKs in response to oxidative stress. Blocking of the survival signaling mediated by FAM120A, which sensitizes the cancer cells to stress-induced apoptosis, may be a novel therapeutic approach for gastric scirrhous carcinoma cells.
~130 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: N-terminus
KLH-conjugated synthetic peptide corresponding to the N-terminus of human FAM120A.
Application
Anti-FAM120A Antibody is an antibody against FAM120A for use in western blotting, IHC & immunofluorescence.
Immunohistochemistry Analysis: 2.5 μg/mL from a representative lot detected FAM120A in rat brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected FAM120A in rat brain cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Biochem/physiol Actions
This antibody will not cross-reacti with FAM120B/PGCC1.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in SK-N-SH cell lysate.
Western Blotting Analysis: 0.5 μg/mL of this antibody detected FAM120A in SK-N-SH cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
