Description
General description
PKA RII Beta is a type two regulatory chain which mediates membrane association by binding to anchoring proteins, including the MAP2 kinase. When the enzyme is inactive there are two regulatory chains and two catalytic chains. PKA RII Beta is activated by cAMP and produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
~ 46 kDa observed
Immunogen
Epitope: cAMP2 domain
KLH-conjugated linear peptide corresponding to human PKA RII Beta at the cAMP2 domain.
Application
Anti-PKA RII Beta Antibody is an antibody against PKA RII Beta for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot Analysis: 0.5 µg/mL from a previous lot detected PKA RII Beta on 10 µg of mouse brain tissue lysate.
Biochem/physiol Actions
This antibody recognizes PKA RII Beta at the cAMP2 domain.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human brain tissue lysate
Evaluated by Western Blot in human brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PKA RII Beta on 10 µg of human brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
