Description
General description
LAT (Linker for activation of T cells) is a transmembrane adaptor protein that plays an essential role in T cell receptor (TCR) signal transduction pathways. LAT is phosphorylated on multiple tyrosine residues upon TCR engagement, recruits several important signaling proteins to the membrane, and functions as a scaffold for the assembly of numerous signaling complexes.
~36 kDa observed. Uncharacterized bands may be observed in some cell lysates.
Immunogen
Epitope: Cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1
KLH-conjugated linear peptide corresponding to the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of human LAT phosphorylated at Tyrosine 200.
Application
Detect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.
Research Category
Signaling
Research Sub Category
Immunological Signaling
Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho LAT (Tyr200) in CD3 treated Jurkat cell lysates. However, a decrease in signal was observed against phospho LAT (Tyr200) in untreated Jurkat cell lysates.
Biochem/physiol Actions
Other homologies: Rat, Mouse, and Bovine (89% sequence homology).
This antibody recognizes the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of LAT phosphorylated at Tyrosine 200.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.
Evaluated by Western Blotting in LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho LAT (Tyr200) immunoprecipitated from CD treated Jurkat cell lysates. A decrease in signal was observed against phospho LAT (Tyr200) immunoprecipitated from untreated Jurkat cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-467
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
