Description
General description
Arginase-1, the protein encoded by the ARG1 gene, is the enzyme that catalyzes the breakdown of L-arginine into ornithine and urea. ARG1 is a key player in the Urea cycle. There are two distinct isoforms of Arginase, Arginase I and Arginase II. Arginase I (also called the liver type arginase or Type 1 arginase) is a highly expressed cytosolic enzyme in the liver and other tissues, including the brain. Arginase II or kidney-type arginase is also widely expressed at low levels in many tissues but localized to the mitochondria primarily. Interestingly, Arginase 1 has neuroprotective properties. Arginase I is essential for protecting motor neurons from nitric oxide dependent apoptosis that can be induced upon cellular stress or trophic factor removal. By suppressing nitric oxide production, Arginase prevents the formation of peroxynitrite and its subsequent cellular toxicity. Furthermore, Arginase 1 appears to play critical roles throughout the CNS for survival and regeneration of neurons and axons. In cancer, Arginase 1, already a useful marker for hepatocytes is also an excellent marker for hepatocellular cancers.
~ 38 kDa observed
Immunogen
Full-length recombinant protein corresponding to Rat Arginase-1.
Application
Immunohistochemistry Analysis: A representative lot detected Arginase-1 in bronchoalveolar lavage cells (Zimmermann, N., et al. (2003). J. Clin. Invest. 111:1863–1874 (2003)).
Western Blotting Analysis: A representative lot detected Arginase-1 in RAW cell lysate (Morris, S., et al. (1998). Am J Physiol Endocrinol Metab 275:E740-E747, 1998).
Research Category
Signaling
This Anti-Arginase-1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Arginase-1.
Physical form
Format: Purified
IgY purified from Yolk
Purified chicken Polyclonal other in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse LN tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Arginase-1 in 10 µg of mouse LN tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
