Description
General description
Periostin, also known as PN, or Osteoblast-specific factor 2 (OSF-2), and encoded by the gene POSTN/OSF2, is a secreted extracellular matrix protein that appears to be involved in inducing cell attachment and spreading as well as cell adhesion. In bone, Periostin enhances mineralization and assists in the incorporation of BMP1 protein into the fibronectin extracellular matrix of connective tissues. Periostin is preferentially expressed in periosteum and periodontal ligaments but can also be found expressed in developing heart and other tissues. Periostin is also over expressed in many cancers where is contributes to a growth-supportive role for such cancers and also aids in chemoresistance for cancers cells through the activation of the PI3K/Akt/surviving pathway.
~75-85 kDa observed. Uniprot describes five isoforms at 93 kDa, 90 kDa, 90 kDa, 87 Kda, and 87 kDa However, this protein has been observed between 75-85 kDa
Immunogen
Linear peptide from the fasciclin 1 domain of mouse periostin.
Application
Anti-Periostin (Pan) Antibody is an antibody against Periostin (Pan) for use in western blotting & IHC.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western Blotting Analysis: A representative lot detected Periostin (Pan) in mouse lung extract.
Immunohistochemistry Analysis: A representative lot detected Periostin (Pan) in certain reported tissues (Kruzynska-Frejtag, A., et al. (2004). Dev Dyn. 229(4):857-868.; Butcher, J. T., et al. (2007). Dev Biol. 302(1):256-266.; Norris, R. A., et al. (2007). J Cell Biochem. 101(3):695-711.; Inai, K., et al. (2008). Dev Biol. 315(2):383-396.; Norris, R. A., et al. (2008). Dev Biol. 316(2):200-213.).
Biochem/physiol Actions
This antibody detects all splice variants of Periostin.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing PBS.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse lung tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Periostin (Pan) in 10 µg of mouse lung tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
