Description
Application
Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).
Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.
Studies on HCAM (homing receptor) function.
Immunoprecipitation.
Optimal working dilutions must be determined by the end user.
This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.
Physical form
Format: Purified
Analysis Note
Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
