Description
Immunogen
Whole human thymic stoma cells (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).
Application
Immunohistochemistry Analysis: A representative lot immunostained fibroblastic cells in paraffin-embedded gastrocnemius section from a patient with myopathic condition (Courtesy of Duy Minh Ha, University of Nebraska Medical Center, Omaha, NE)
Immunocytochemistry Analysis: A representative lot immunostained human muscle fibroblasts by fluorescent immunocytochemistry (Agley, C.C., et al. (2015). J. Vis. Exp. (95):52049).
Immunocytochemistry Analysis: A representative lot immunostained MRC-5 fetal fibroblasts and normal human dermal fibroblasts (NHDF), but not fibrocytes or macrophages by immunocytochemistry (Bertolotto, C., et al. (2009). PLoS One. 4(10):e7475).
Flow Cytometry Analysis: A representative lot detected fibroblasts differentiated from human ESCs and iPSCs (Zhang, J., et al. (2012). Circ. Res. 111(9):1125-1136).
Immunofluorescence Analysis: A representative lot immunostained fibroblasts in human skeletal muscle cryosections by fluorescent immunohistochemistry (Agley, C.C., et al. (2013). J. Cell. Sci. 126(Pt 24):5610-5625).
Immunofluorescence Analysis: A representative lot immunostained the fibrous stroma and vessels in acetone-fixed, frozen human tissue sections by fluorescent immunohistochemistry (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Fibroblasts Antibody, clone TE is validated for use in Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF) and Immunohistochemistry (IHC) for the detection of Fibroblasts.
Biochem/physiol Actions
The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
Physical form
Format: Purified
The monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Preparation Note
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
