Description
General description
Endothelin consists of a family of potent vasoconstrictor peptides, which include four structurally related isoforms, ET-1, ET-2, ET-3 and vasointestinal contractor (VIC, β -ET)
Immunogen
endothelin-1 (ET-1) conjugated to KLH
Application
Monoclonal Anti-Endothelin-1 antibody produced in mouse is suitable for immunoblotting and immunocytochemistry.It is also suitable for radioimmunoassay (RIA) at a working dilution of 1:25,000 and for immunohistochemistry at 1:250 working dilution using formalin-fixed, paraffin-embedded tissue sections.
Biochem/physiol Actions
ET-1, a 21 amino acid peptide, is produced by vascular endothelial cells. It is a potent vasoconstrictor and plays an important role in the homeostasis of the circulatory system. It is also important in pathogenesis of cardiovascular diseases. It is also involved in contraction of airway and intestinal smooth muscle. It releases vasodilator prostaglandins and nitric oxide (NO). It also plays a role in mitogenic effects on vascular smooth muscle cells and fibroblasts, stimulation of atrial natriuretic peptide secretion from atrial cardiocytes and inhibition of renin release. ET-1 is expressed in the spinal cord and dorsal root ganglia, where it may serve as a neurotransmitter/neuromodulator.
The antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.
Physical form
Solution in phosphate buffered saline, containing 0.05% sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
