Description
General description
Brain derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions.
Product Source: E. coli
Application
Research Category
Neuroscience
Research Sub Category
Growth Factors & Receptors
Biochem/physiol Actions
Cross Reactivty
Chicken, Frog, Mouse, Rat, Hamster, Guinea Pig, and Monkey
Physical form
Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human BDNF is manufactured using all non-animal reagents
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Bioactivity assay: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
