Description
General description
16 kDa calculated
Angiogenin (Ang) is a non-glycosylated polypeptide, 123 amino acids in length, with a molecular mass of 14 kDa. It is secreted by tumor cells and is a potent inducer of neovascularization. Angiogenin is produced by a variety of cell types including vascular endothelial cells and smooth muscle cells, fibroblasts, tumor colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. It specifically binds to endothelial cells and elicits second messenger systems. Angiogenin is present in human follicular fluid, and its production is up-regulated by human chorionic gonadotropin and hypoxic conditions. Angiogenin shows a high degree of homology with known ribonucleases such as pancreatic ribonuclease A, and the capacity of angiogenin to induce blood vessel growth is dependent on its ribo-nucleolytic activity. Angiogenin is thought to be involved in the development of solid tumors; angiogenin antagonists are capable of inhibiting tumor growth. Angiogenin is endocytosed by subconfluent endothelial cells and translocated to the nucleus, where it accumulates in the nucleolus. Serum angiogenin concentration is increased in serum of colorectal cancer patients, and correlates with cancer progression. The cytoskeletal protein α-actinin-2 binds to angiogenin, which might be a novel interface target for anti-angiogenesis and anti-tumor therapy.
Immunogen
Purified Human Angiogenin.
Application
Inhibition Assay: A representative lot of this antibody blocked cell-free protein synthesis by angiogenin (Fett et al., (1994) Biochemistry. 33(18):5421-5427).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Angiogenin Antibody, clone 26–2F (Azide Free) is validated for use in Immunocytochemistry and Inhibition and Immunohistochemistry for the detection of Angiogenin.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
