Description
General description
The Wnt family of cysteine-rich proteins constitutes a group of ligands known for their roles in development such as cell differentiation, proliferation, migration and embryonic patterning. They have also been implicated in the formation of tumors. Wnts are involved in the stimulation of several signaling cascades that have been divided into two types; the canonical pathway, and the non-canonical pathway. Wnt-5a is considered to be a non-canonical ligand for the Wnt signaling pathway and is a ligand to the frizzled family of seven transmembrane receptors. When interacting with ROR2, Wnt-5a is considered to inhibit tumor formation due to Wnt-5a’s suppression of the canonical Wnt signaling pathway. Wnt-5a is involved in activating cell migration, regulating the motility of melanoma cells, decreasing the proliferation, chondrogenesis, and migration of carcinoma cells, and inhibiting the expression of type II collagen in chondrocytes. Wnt-5a is a requirement to the endothelial differentiation and vascularization of Embryonic Stem Cells, and is also necessary to the development of mammary glands, limb outgrowth, and the genital tubercle.
~70 kDa observed
Immunogen
Purified recombinant fragment of human Wnt-5a expressed in E. Coli.
Application
Detect Wnt-5a using this mouse monoclonal antibody, Anti-Wnt-5a Antibody, clone 6F2 validated for use in western blotting, Immunofluorescence & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected Wnt-5a in human lung cancer, thyroid cancer, lymph node, and brain tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Wnt-5a in PC-12 cells.
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Wnt-5a-hIgGFc transfected HEK293 cell lysate
Evaluated by Western Blotting in Wnt-5a-hIgGFc transfected HEK293 cell lysate.
Western Blotting Analysis: A 1:500-2000 dilution of this antibody detected Wnt-5a in Wnt-5a-hIgGFc transfected HEK293 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
