General descriptionMesenchymal stem cells, also known as marrow stromal cells, are defined as a self-renewing population of adherent, bone-marrow-derived multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined cultures in vitro, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissues.
EMD Millipore’s Human Mesenchymal Stem Cells (Bone Marrow) are isolated from normal human bone marrow and are cryopreserved at P2 to ensure optimal phenotype and the highest viability and plating efficiency. These cells proliferate as an adherent cell monolayer and have shown a strong capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes..
ApplicationResearch Category
Stem Cell Research
Biochem/physiol ActionsMesenchymal Stem Cells
Preparation NoteHuman Mesenchymal Stem Cells (Bone Marrow) should be stored in liquid nitrogen. The cells are guaranteed for at least 3 population doublings without significantly affecting cell surface marker expression and differentiation potential.
Analysis Note- Each vial contains at least 1X10⁶ viable cells at P2.
- Cell viability is greater than 75% at thaw and cells can be cultured for at least 3 population doublings after the initial thaw.
- Bone marrow donors tested negative for HIV, hepatitis B and hepatitis C.
- Apparently normal karyotype per cytogenetic analysis.
- Cells tested negative for mycoplasma contamination.
Other Notes1X10⁶ viable Human Bone Marrow Mesenchymal Stem Cells
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerRESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
