General descriptionThe YUMMER1.7D4 mouse melanoma cell line is both immunocompetent and reflective of the somatic mutations common in melanomas. YUMMER1.7D4 carries three driver mutations of melanoma: Braf V600E, Pten -/- and Cdkn2 -/- (1,2). In addition, the YUMMER1.7D4 cell line harbors a high frequency of stable UV-induced somatic mutations which have been shown to stimulate host adaptive immune response (3). YUMMER1.7D4 cells are diploid, allowing for enhanced knockout frequency in CRISPR-based screens. The unique features of the YUMMER1.7D4 cell line make it a valuable model for studies of immune checkpoint inhibition and mechanisms of anti-tumor responses.
Source:
The YUMMER1.7D4 mouse melanoma cell line is a diploid clonal isolate from YUMM1.7D4 cells exposed to UVB radiation. The original YUMM1.7 cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57/B1/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing (1). The YUMMER1.7D4 cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten and Cdkn2 (3).
Research Category:
Cancer
Biochem/physiol ActionsMouse, Cancer Cells
Packaging≥1X10⁶ cells/vial
Preparation NoteStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other NotesSubject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerThis product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
