Description
General description
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.
The RIPAb+ PABPC1 set includes the PABPC1 antibody, a negative control antibody (purified mouse IgG), and positive qPCR primers which amplify a 100 bp fragment of the human ACTB cDNA. The PABPC1 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of PABPC1-associated RNAs.
Immunogen
The PABPC1 monoclonal antibody is made against a fusion protein corresponding to full-length of human cytoplasmic poly (A)-binding protein (PABPC1).
Application
This RIPAb+ PABPC1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Immunoprecipitation from RIP lysate:
RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP
Analysis Note
RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 x 10⁷cell equivalents per IP) were subjected to immunoprecipitation using 5 ug of either a normal mouse IgG or the anti-PABPC1 antibody and the Magna RIP Kit (Cat. # 17-700).
Successful immunoprecipitation of PABPC1-associated RNA was verified by qRT-PCR using RIP Primers, ACTB. Please refer to the Magna RIP (Cat. # 17-700) or EZ-Magna RIP (Cat. # 17-701) protocol for experimental details.
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
