Description
General description
Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.
Application
Cellulase from Aspergillus niger has been used:
in the preparation of the standardized solution to determine absolute cellulase levels
in phosphate-citric acid buffer for activity assay
in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed
Biochem/physiol Actions
Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
Other Notes
1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
