Description
General description
Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.
Application
DL-dithiothreitol solution has been used:
in the treatment of intestinal explants
in homogenate preparation of gill cells for enzymatic assays
in protein electrophoresis for western blotting
in transglutaminase activity
in sample buffer for SDS-PAGE
in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
as a component of protein extraction buffer for cell lysis
Biochem/physiol Actions
DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.
Features and Benefits
BioUltra grade - 1M DTT solution, suitable for molecular biology
Free from DNases, RNases, phosphatases, and proteases
Other Notes
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