Description
General description
Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending
Pellet Paint NF Co-Precipitant is a nonfluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye Terminators during the alcohol precipitation of cycle sequencingreaction products. Cycle sequencing reactions can be precipitated rapidly with 1 µl of carrierper reaction and centrifugation times of 10minutes. The easily visualized carrier providesa simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labeled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Resuspension of pelleted sequencing reaction products in deionized formamide can beconfirmed by checking for dissolution of the carrier. Pellet Paint NF Co-Precipitantis fully compatible with the ABI PRISM®BigDye Terminator Cycle Sequencing Ready Reaction. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer before the cycle sequencing reaction. Although PelletPaint NF absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet PaintNF) can be used as a correction factor when determining nucleic acid concentration. PelletPaint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequence accuracy. Pellet Paint NF Co-Precipitant is a useful substitute for the original Pellet Paint Co-Precipitant in applications where fluorescent detection is used.
Features and Benefits
Efficient and rapid precipitation of BigDye® cycle sequencing products
Efficient removal of dye terminators
Direct visualization and tracking of precipitated material
No effect on sequencing reaction
Substitute for original Pellet Paint Co-Precipitant for fluorescent detection applications
Other Notes
•: 125 µl or 1 ml: Pellet Paint NF Co-Precipitant
•: 1 ml or 8 ml: 3 M Sodium Acetate, pH 5.2
Legal Information
This product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates.
ABI Prism is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
BigDye is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PELLET PAINT is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
