Merck, E3024-500ML, Earle′s Balanced Salts

General description
Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).
Application
Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.