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Merck, E3763-1MG, Resorufin ethyl ether

Size:1 mg, 7-Ethoxy-3H-phenoxazin-3-one, Ethoxyresorufin, O⁷-Ethylresorufin, ≥98% (TLC), powder
Available Catalog Numbers
E3763-1MGE3763-5MG
Catalog Number: E3763-1MG
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Category
ELISA Substrates
Pack Size1 mg
CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & BioproductionMerck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification
Manufacturer's Pagewww.sigmaaldrich.com/US/en/product/SIGMA/E3763
Description
General description
Fluorimetric substrate for cytochrome P450 linked enzyme, CXPIA1/2
Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.
Resorufin is the reduced product of resazurin, also termed as Alamar Blu. It exhibits fluorescence at longer wavelengths and is flexible for modification in its hydroxyl group.

Research area: Cell Signaling
Application
Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors. It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).
Specifications

General

Web Name Suffix Override≥98% (TLC), CXPIA1/2 substrate, powder
NACRESNA.47
PubChem Substance ID24894527
UNSPSC Code12161501
MDL numberMFCD00037661
Beilstein/REAXYS Number225973
InChI1S/C23H14F5NO4.C14H11NO3/c24-17-18(25)20(27)22(21(28)19(17)26)33-16(30)9-29-23(31)32-10-15-13-7-3-1-5-11(13)12-6-2-4-8-14(12)15;1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h1-8,15H,9-10H2,(H,29,31);3-8H,2H2,1H3
SMILES stringCCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1.Fc4c(F)c(F)c(OC(=O)CNC(=O)OCC5c6ccccc6-c7ccccc57)c(F)c4F
InChI keyZOSYTBPPLWBBKM-UHFFFAOYSA-N
assay≥98% (TLC)
formpowder
storage condition(Tightly closed. Dry)
technique(s)activity assay: suitable
colororange to red
mp223-225 °C (lit.)
solubilitychloroform: 9.80-10.20 mg/mL, clear, orange
suitabilitysuitable for fluorescence
storage temp.−20°C
Quality Level200