ApplicationEosin Y has been used as a stain:
- to determine the location of the brain sections
- for staining of fixed liver tissue
- for histological examination of brain sections from the dorsal hippocampus
Biochem/physiol ActionsEosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.
