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Merck, E5134-100G, Ethylenediaminetetraacetic acid disodium salt dihydrate

Size:100 g, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂, Sequestrene Na₂, suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)
Available Catalog Numbers
E5134-100GE5134-1KGE5134-250GE5134-500GE5134-50GE5134-5KG
Catalog Number: E5134-100G
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Category
Biological Buffers
Pack Size100 g
TagsBiological BuffersChemistry & BiochemicalsBiochemicals
CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & BuffersMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & BioproductionMerck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification
Manufacturer's Pagewww.sigmaaldrich.com/US/en/product/SIGMA/E5134
Description
General description
EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests
Application
EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
  • an anticoagulant for whole blood collection
  • a stabilizer solution for freezing cell culture samples
  • in combination with PBS and trypsin, to trypsinize cells
  • for phytoextraction of heavy metals in polluted soils

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Features and Benefits
  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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Specifications

General

NACRESNA.31
PubChem Substance ID24894605
UNSPSC Code12352107
EC Number205-358-3
Beilstein/REAXYS Number3900609
InChI keyOVBJJZOQPCKUOR-UHFFFAOYSA-L
InChI1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
SMILES stringO.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
gradeMolecular Biology
assay99.0-101.0% (titration)
formpowder
reaction suitabilityreagent type: chelator
technique(s)electrophoresis: suitable
impurities≤0.005% Insolubles
mp248 °C (dec.) (lit.)
solubility3 M NaOH: 1.6 g/10 mL
cation tracesFe: ≤0.005%
suitabilitysuitable for electrophoresis
foreign activityDNase, RNase, NICKase and protease, none detected
storage temp.room temp
Quality Level300