Description
General description
Hippocalcin-like protein 1 (UniProt: P37235; also known as Calcium-binding protein BDR-1, HLP2, Visinin-like protein 3, VILIP-3) is encoded by the HPCAL1 (also known a BDR1) gene (Gene ID: 3241) in human. HPCAL1 is a lipid-anchor membrane protein of the visinin-like subfamily of neural calcium sensor and is involved in the calcium-dependent regulation of rhodopsin phosphorylation. It is structurally related to Ca2+-myristoyl switch proteins that contain four EF-hand motifs and a covalently attached N-terminal myristoyl group. HPCAL1 is also expressed in Purkinje cells of the cerebellum and hippocampus where it is believed to regulate synaptic plasticity relevant for learning and memory. Clone 1E10 specifically detects denatured HPCAL1 protein that is endogenously expressed in several pancreatic cell lines. (Ref.: Chen, Y., et al. (2014). Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(1); 20-27; Li, C., et al. (2016). PLoS One 11(11); e0165921).
~22 kDa observed; 22.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified native and denatured human HPCAL1.
Application
Detects Hippocalcin-like protein 1 using this mouse monoclonal Anti-HPCAL1, clone 1E10, Cat. No. MABC1139, tested for use in Western Blotting and Immunoprecipitation.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected HPCAL1 in Western Blotting applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).
Immunoprecipitation Analysis: A representative lot detected HPCAL1 in Immunoprecipitation applications (Chen, Y., et. al. (2014). Monoclon Antib Immunodiagn Immunother. 33(1):20-7).
Biochem/physiol Actions
Clone 1E10 specifically detects Hippocalcin-like protein 1 (HPCAL1) in human cells.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in Panc-1 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected HPCAL1 in Panc-1 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
