Merck, S9012-25G, Sulforhodamine B sodium salt
Size:25 g, Acid red 52; Acid Rhodamine B, Technical grade
Available Catalog Numbers
Catalog Number: S9012-25G
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Category
Pack Size25 g
Description
General description
Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.
Application
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.
Application
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
Specifications
General
| UNSPSC Code | 12171500 |
|---|---|
| Web Name Suffix Override | Powder |
| NACRES | NA.47 |
| PubChem Substance ID | 24899824 |
| EC Number | 222-529-8 |
| MDL number | MFCD00010180 |
| Colour Index Number | 45100 |
| Beilstein/REAXYS Number | 3886008 |
| InChI | 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1 |
| InChI key | SXQCTESRRZBPHJ-UHFFFAOYSA-M |
| SMILES string | [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1 |
| form | powder |
| composition | Dye content, ~75% |
| ε<sub>max</sub> | ≥85000 at 554-560 nm |
| application(s) | diagnostic assay manufacturinghematologyhistology |
| storage temp. | room temp |
| Quality Level | 200 |
