Description
Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Specifications

General

UNSPSC Code12352111
NACRESNA.31
PubChem Substance ID24900617
EC Number200-315-5
Beilstein/REAXYS Number635724
MDL numberMFCD00008022
InChI keyXSQUKJJJFZCRTK-UHFFFAOYSA-N
InChI1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
SMILES stringNC(N)=O
product lineBioXtra
assay98.5-101.5%
formpowder, crystals or pellets
impurities≤0.005% Phosphorus (P)
ign. residue≤0.1%
pH7.5-9.5 (20 °C, 5 M in H2O)
Quality Level200
Gene Informationhuman ... CA1(759), CA2(760) rat ... Ppm1a(24666)
mp132-135 °C (lit.)
solubilityH2O: 5 M at 20 °C, clear, colorless
density1.335 g/mL at 25 °C (lit.)
anion traceschloride (Cl-): ≤0.05%
cation tracesAl: ≤0.0005%
absorption≤0.06 at 260 nm at 5 M
application(s)diagnostic assay manufacturing
Merck U0631-1KG Urea | IRIGHT