Merck, U211200-100G, DMT-2′Fluoro-dU Phosphoramidite
Size:100 g, DMT-2′-Fluoro-dU amidite, ≥99.0% (reversed phase HPLC)
Available Catalog Numbers
Catalog Number: U211200-100G
Request a quote.
Category
Pack Size100 g
Description
General description
DMT-2′Fluoro-dU Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.
Its key features are-
Application
DMT-2′Fluoro-dU Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry.
DMT-2′Fluoro-dU Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.
Its key features are-
- Can be employed together with DNA or RNA phosphoramidites
- Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
- Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
- Consistent lot-to-lot high purity and performance
- Manufactured under a certified ISO 9001 quality system
Application
DMT-2′Fluoro-dU Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry.
Specifications
General
| NACRES | NA.51 |
|---|---|
| PubChem Substance ID | 329827613 |
| UNSPSC Code | 41116105 |
| MDL number | MFCD11113919 |
| InChI | 1S/C39H46FN4O8P/c1-26(2)44(27(3)4)53(50-24-10-22-41)52-36-33(51-37(35(36)40)43-23-21-34(45)42-38(43)46)25-49-39(28-11-8-7-9-12-28,29-13-17-31(47-5)18-14-29)30-15-19-32(48-6)20-16-30/h7-9,11-21,23,26-27,33,35-37H,10,24-25H2,1-6H3,(H,42,45,46)/t33-,35-,36-,37-,53?/m1/s1 |
| SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5 |
| InChI key | HQHQPAYRJJMYQX-DJTPZYMWSA-N |
| biological source | non-animal source (no BSE/TSE risk) |
| product line | Proligo Reagents |
| assay | ≥99% (31P-NMR) |
| form | powder |
| mol wt | 748.78 g/mol |
| technique(s) | oligo synthesis: suitable |
| impurities | ≤0.3% water content (Karl Fischer) |
| color | white to off-white |
| <i>λ</i> | conforms (UV/VIS Identity) |
| suitability | conforms to structure for H-NMR |
| nucleoside profile | base: deoxyuridinebase protecting group: none2' protecting group: fluoro5' protecting group: DMTdeprotection: fast/standard |
