Merck, U5378-100G, Urea

Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

• Urea has been used in protein resuspension buffer and to denature proteins.
• It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
• It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.